Engineered β-Lactoglobulin Produced in E. coli: Purification, Biophysical and Structural Characterisation
نویسندگان
چکیده
منابع مشابه
Engineered β-Lactoglobulin Produced in E. coli: Purification, Biophysical and Structural Characterisation
Functional recombinant bovine β-lactoglobulin has been produced by expression in E. coli using an engineered protein gene and purified to homogeneity by applying a new protocol. Mutations L1A/I2S introduced into the protein sequence greatly facilitate in vivo cleavage of the N-terminal methionine, allowing correctly folded and soluble protein suitable for biochemical, biophysical and structural...
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E. coli heat stable enterotoxin STa is an agonist of the membrane guanylate cyclase C whose endogenous ligands are the peptide hormones guanylin and uroguanylin. Whereas these peptides contain only two disulfide bonds, STa is stabilized by one additional disulfide bridge. We chemically synthesized the enterotoxin STh that originates from the E. coli strain found in humans, and we determined its...
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ژورنال
عنوان ژورنال: Molecular Biotechnology
سال: 2016
ISSN: 1073-6085,1559-0305
DOI: 10.1007/s12033-016-9960-z